Recombinant Human Syndecan-4

Molecule:

SDC4

Synonyms:

SYND4, SDC4

Species:

Human

Cat. No.:

CSI20111A pdf (datasheet)

Quantity/Size:

10 μg

Price:

$190.00 BUY

Description:

Recombinant Human Syndecan-4 is a single non-glycosylated polypeptide chain containing 127 amino acids.

Background: Syndecan-4 (SDC-4) also known as amphiglycan or ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan4 is the most similar to Syndecan2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGFβ2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator. Human Syndecan4 ECD shares approximately 79%, 78% and 81% aa identity with mouse, rat and porcine Syndecan4 ECD, respectively.

Entrez Symbol:

SDC4

Data PDF:

CSI20111

Source:

E. coli

Molecular Weight:

~13.9 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Purity:

>95% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The specific activity is determined by binding ability in a functional ELISA. Immobilized rHuSDC-4 at 500 ng/ml (100 μl/well) can bind rHuFGF basic with a linear range of 0.1-10 ng/ml.

Endotoxin Level:

Less than 1EU/μg of rHuSDC-4 as determined by LAL method.

Amino Acid Sequence:

ESIRETEVID PQDLLEGRYF SGALPDDEDV VGPGQESDDF ELSGSGDLDD LEDSMIGPEV VHPLVPLDNH IPERAGSGSQ VPTEPKKLEE NEVIPKRISP VEESEDVSNK VSMSSTVQGS NIFERTE

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.