Recombinant Human Thrombopoietin

Molecule:

THPO

Synonyms:

TPO, MGDF, MKCSF, ML, MPLLG, THCYT1

Cat. No.:

CRT400C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$5,100.00 BUY

Description:

Recombinant human THPO, produced in E. coli, is a single polypeptide chain containing 175 amino acids.

Background: Thrombopoietin (THPO), the ligand for the receptor encoded by the c-Mpl proto-oncogene, is a key regulator of megakaryocytopoiesis (the development process that leads to platelet production) and thrombopoiesis in vitro and in vivo. Thrombopoietin is produced by liver and kidney tissue. Recombinant THPO has now been shown to stimulate the maturation, as well as the proliferation, of megakaryocytes and may have important therapeutic applications for the treatment of various clinical conditions associated with thrombocytopenia. 

Entrez Symbol:

THPO

Uniprot Name:

thrombopoietin

Uniprot ID:

P40225

Data PDF:

CRT400

Source:

E. coli

Molecular Weight:

18.8 kDa

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5.

Purity:

>90% by SDS-PAGE

Biological Activity:

Activity, as determined by the dose-dependent proliferation of human MO7e cells is < 5 ng/ml.

Specific Activity:

>2 × 105 U/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MSPAPPACDL RVLSKLLRDS HVLHSRLSQC PEVHPLPTPV LLPAVDFSLG EWKTQMEETK AQDILGAVTL LLEGVMAARG QLGPTCLSSL LGQLSGQVRL LLGALQSLLG TQLPPQGRTT AHKDPNAIFL SFQHLLRGKV RFLMLVGGST LCVRRAPPTT AVPSRTSLVL TLNEL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

On receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.