Recombinant Human TNF-alpha

Molecule:

TNF-alpha

Synonyms:

TNF-alpha, TNFA, TNFSF2, DIF

Species:

Human

Cat. No.:

CRT100C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,200.00 / Special Price: $965.00 BUY

Description:

Tumor necrosis factor alpha (TNF-alpha) is a multifunctional proinflammatory cytokine that belongs to the TNF superfamily. TNF-alpha is secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their stimulation by bacterial LPS. TNF-alpha activates signals through two receptors, TNFR1, which is expressed on most cell types, and TNFR2, which is expressed mainly on immune cells. TNF-alpha can have many functions including stimulation of phagocytosis in macrophages, chemoattraction of neutrophils, increase in insulin resistance, and inducing fever, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Recombinant Human TNF-alpha is a single, non-glycosylated polypeptide chain containing 158 amino acids.

Entrez Symbol:

TNF

Uniprot Name:

Tumor necrosis factor

Uniprot ID:

P01375

Data PDF:

CRT100

Source:

E. coli

Molecular Weight:

~17.5 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered solution in 10 mM sodium phosphate, pH 7.5.

Purity:

>95% by HPLC and SDS-PAGE

Biological Activity:

As determined by a cytotoxicity assay using mouse L929 cells in the presence of Actinomycin D, is < 0.05 ng/ml.

Specific Activity:

>1 x 107 U/mg

Endotoxin Level:

<1 EU/µg of TNF alpha as determined by LAL method

Amino Acid Sequence:

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C. After reconstitution, the preparation is stable for up to one month at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots in a 0.1% BSA solution and store at -20°C to -80°C