Recombinant Human soluble RANK Ligand

Molecule:

RANKL

Synonyms:

ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, soluble Receptor Activator of NFkB Ligand, TNF-related activation-induced cytokine, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kapp

Species:

Human

Cat. No.:

CRR100B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Receptor Activator of NF-?B Ligand (RANKL) is a cell-bound marker (CD254) related to the TNF family of proteins. RANKL plays a critical role in bone metabolism, particularly osteoclast differentiation. In addition, RANKL is expressed by some T cells and is thought to promote dendritic cell maturation. Recombinant Human soluble RANKL is a single, non-glycosylated polypeptide chain containing 176 amino acids.

Entrez Symbol:

TNFSF11

Uniprot Name:

Tumor necrosis factor ligand superfamily member 11

Uniprot ID:

O14788

Data PDF:

CRR100

Source:

E. coli

Molecular Weight:

20 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered 1 mg/ml solution containing 10 mM Na2PO4, pH 8.0.

Purity:

Err:509

Biological Activity:

Biologically active when compared to standard. The ED50 as determined by its ability to induce a dose-dependent stimulation of IL-8 production by human PBMCs is <100 ng/ml.

Endotoxin Level:

0.20 EU/οΨΒg of protein (with a 50% confidence range)

Amino Acid Sequence:

MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID

Reconstitution:

Centrifuge vial prior to opening. First add sterile distilled water to the vial to fully solubilize the protein to a concentration not less than 100 οΨΒg/ml. Gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solu

Storage/Stability:

Lyophilized Lyophilized product is stable at room temperature for shipping purposes. After receipt, store at -20οΨΑC. Reconstituted material should be aliquoted and frozen at -20οΨΑC. It is recommended that a carrier protein such as 0.1% HSA or BSA is add