Recombinant Human UBC9 His

Molecule:

UBC9

Synonyms:

SUMO-protein ligase, SUMO-1-protein ligase, ubiquitin-protein ligase I, ubiquitin carrier protein 9, ubiquitin carrier protein I, ubiquitin-protein ligase E2I, ubiquitin conjugating enzyme 9, ubiquitin-conjugating enzyme E2 I, ubiquitin-conjugating enzyme UbcE2A,

Species:

Human

Cat. No.:

CSI12723 pdf (datasheet)

Quantity/Size:

10 μg

Price:

$190.00 BUY

Description:

Background: Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiq

Entrez Symbol:

UBE2I

Uniprot Name:

SUMO-conjugating enzyme UBC9

Uniprot ID:

P63279

EC number:

6.3.2.19

Data PDF:

CSI12723

Source:

E. coli

Molecular Weight:

~19.5 kDa

Tag:

His

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered (through a 0.22 �m filter) concentrated (1 mg/ml) solution in 1� PBS, pH 7.5 + 1 mM DTT.

Purity:

>95% by SDS-PAGE and HPLC analyses.

Endotoxin Level:

<0.1 ng/�g

Amino Acid Sequence:

HHHHHHAMGTLNMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of 0.1-1.0 mg/ml. The solution can then be further diluted in other aqueous buffers.

Storage/Stability:

Lyophilized protein is stable at 2-4℃C, but is preferably stored desiccated at -20℃C. After reconstitution, stable for ?1 week at 2-4℃C. For long term storage, aliquot and store at -20℃C to -80℃C. Avoid repeated freeze-thaw c