Recombinant Mouse CD40 Ligand

Molecule:

CD40 Ligand/TNFSF5

Synonyms:

IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b

Species:

Mouse

Cat. No.:

CRC803C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 BUY

Description:

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2. Recombinant mouse CD40 Ligand is a non-glycosylated protein, containing the 149 C-terminal amino acids.

Entrez Symbol:

Cd40lg

Data PDF:

CRC803

Source:

E. coli

Molecular Weight:

16.4 kDa

Formulation:

Recombinant mouse CD40 Ligand is lyophilized from 10 mM Na2PO4, pH 7.5 and 0.1 M Arginine.

Purity:

> 98.0% as determined by HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm

Biological Activity:

The activity is determined by the dose production of IL-8 by human PBMCs and is typically 5-10 ng/mL.

Endotoxin Level:

Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.

Amino Acid Sequence:

MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL

Reconstitution:

Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a co

Storage/Stability:

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.