Recombinant Mouse Exodus-2/CCL21

Molecule:

CCL21

Synonyms:

Chemokine (C-C motif) ligand 21C (leucine), SLC, CKb9, TCA4, Ccl21a, Scya21c, exodus-2, Ccl21c

Species:

Mouse

Cat. No.:

CRE401C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 / Special Price: $1,640.00 BUY

Description:

Recombinant Mouse Exodus 2/CCL21 is a single, non-glycosylated polypeptide chain containing 110 amino acids.

Background: CCL21 is encoded by CCL21 gene that is located in Chr. 9 and shows 21-33 % identity to other CC chemokines. This chemokine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxylterminal domain. CCL21 is highly expressed in lymph nodes of certain endothelial cells, and in the spleen and appendix. It elicits effects by binding with CCR7, having functions of T and B lymphocytes chemotaxis and inhibiting hematopoiesis.

Data PDF:

CRE401

Source:

E. coli

Molecular Weight:

~12.1 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine T-lymphocytes is in a concentration range of 10-100 ng/ml.

Endotoxin Level:

Less than 1 EU/μg of recombinant mouse Exodus-2/CCL21 as determined by LAL method.

Amino Acid Sequence:

SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-4°C. Store at -20°C, preferably desiccated. Upon reconstitution, stable for up to 1 week at 2-4°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.