Recombinant Mouse FGF7/KGF-1

Molecule:

FGF7

Synonyms:

Kgf, Keratinocyte Growth Factor, Fibroblast Growth Factor-7, FGF7, FGF-7, HBGF-7, SDGF-3

Species:

Mouse

Cat. No.:

CRF157B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Recombinant Mouse FGF7/KGF-1 is a single, non-glycosylated polypeptide chain containing 163 amino acids.

Background: Fibroblast Growth Factor-7 (FGF-7/KGF) is one of 23 known members of the FGF family. All FGFs have two conserved cysteine residues and share 30 - 50% sequence identity at the amino acid level. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of variety of tissues, by promoting cellular proliferation and differentiation. KGF-1/FG-7 is a mitogen factor specific for epithelial cells and keratinocytes and signals through FGFR 2b.  KGF-1/FGF-7 plays a role in kidney and lung development, angiogenesis, and wound healing.

Entrez Symbol:

Fgf7

Data PDF:

CRF157

Source:

E. coli

Molecular Weight:

~18.7 kDa

Formulation:

Lyophilized from a 0.2 µm filtered solution in 20 mM PBS, pH 8.0 + 1M NaCl.

Purity:

>96% by SDS-PAGE and HPLC analyses

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.

Endotoxin Level:

Less than 1 EU/µg of rMuKGF-1/FGF-7 as determined by LAL method.

Amino Acid Sequence:

CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -80 °C. Avoid repeated freeze/thaw cycles.