Recombinant Mouse IFN-gamma

Molecule:

IFN-gamma

Synonyms:

IFN-g, IFN-gamma, IFG

Species:

Mouse

Cat. No.:

CRI001C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,000.00 / Special Price: $690.00 BUY

Description:

Recombinant Mouse IFN-gamma is a single non-glycosylated polypeptide chain containing 134 amino acids. Background: Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments TH1 cell expansion and may be required for TH1 cell differentiation.

Entrez Symbol:

Ifng

Uniprot Name:

Interferon gamma

Uniprot ID:

P01580

Data PDF:

CRI001

Source:

E. coli

Molecular Weight:

15.7 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from sterile filtered aqueous solution containing 10 mM sodium phosphate + 50 mM sodium chloride, pH 7.5.

Purity:

>95% by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The specific activity as determined by a CPE assay using EMC virus on L929 cells, is ≥1.25 x 10e7 units/mg

Endotoxin Level:

0.02 EU/µg of protein by kinetic LAL analysis (50% confidence range).

Amino Acid Sequence:

MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized protein is stable at room temperature for shipping purposes. Store desiccated at -20°C on receipt. After reconstitution, stable for ≤1 month at 2-8°C. For long term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.