Recombinant Mouse IL-10

Molecule:

IL-10

Synonyms:

IL-10, IL10, CSIF, Il10

Species:

Mouse

Cat. No.:

CRI154A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$190.00 BUY

Description:

Recombinant Mouse interleukin-10 is a single non-glycosylated polypeptide chain containing 160 amino acids.

Background: IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells.

Entrez Symbol:

Il10

Uniprot Name:

Interleukin-10

Uniprot ID:

P18893

Data PDF:

CRI154

Source:

E. coli

Molecular Weight:

~18.7 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 µm sterile filtered solution of PBS pH 7.4.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.

Endotoxin Level:

Less than 1EU/μg of rMuIL-10 as determined by LAL method.

Amino Acid Sequence:

SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.