Recombinant Mouse Interleukin-11

Molecule:

IL-11

Synonyms:

IL-11

Species:

Mouse

Cat. No.:

CSI20127A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$190.00 BUY

Description:

Recombinant Mouse IL-11 is a a single non-glycosylated polypeptide chain containing 179 amino acids.

Background: Interleukin 11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T11. IL-11 contains no cysteine residues or potential glycosylation sites. IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation.

Entrez Symbol:

Il11

Data PDF:

CSI20127

Source:

E. coli

Molecular Weight:

~19.1 kDa

Formulation:

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of mouse T11 is <2.0 ng/mL.

Specific Activity:

>5 x 105 IU/mg

Endotoxin Level:

Less than 1EU/µg of recombinant mouse IL-11 as determined by LAL method.

Amino Acid Sequence:

MPGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDH SLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLK TLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGS IRAAHAILGG LHLTLDWAVR GLLLLKTRL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.