interleukin-3, mast cell growth factor, mast-cell growth factor, P-cell stimulating factor, P-cell-stimulating factor, hematopoietic growth factor, multilineage-colony-stimulating factor, multipotential colony-stimulating factor
Recombinant Mouse IL-3 is a globular protein containing 134 amino acid residues.
Background: IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon costimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
>98% by SDS-PAGE and HPLC analyses.
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2 of > 2×107 units/mg.
<1 EU/µg of Recombinant Mouse IL-3 as determined by LAL method.
Amino Acid Sequence:
DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in appropriate buffered solutions.
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.