Recombinant Mouse IL-4

Molecule:

IL-4

Synonyms:

Interleukin-4, BSF-1, IL-4

Species:

Mouse

Cat. No.:

CRI129A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$135.00 BUY

Description:

Recombinant mouse IL-4 is a single, non-glycosylated polypeptide chain containing 121 amino acids.

Background: Interleukin-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.

Entrez Symbol:

Il4

Uniprot Name:

Interleukin-4

Uniprot ID:

P07750

Data PDF:

CRI129

Source:

E. coli

Molecular Weight:

13.7 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

>95% by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 as as determined by the dose-dependant prolifiration of Mouse HT-2 cells is less then 20 ng/ml.

Specific Activity:

≥5 x 10e4 U/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.