Recombinant Mouse IL-6

Molecule:

IL-6

Synonyms:

IL-6, HGF, HSF, BSF-2, HP1, BCSF, IFNB2

Species:

Mouse

Cat. No.:

CRI130C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$4,300.00 / Special Price: $2,390.00 BUY

Description:

Recombinant Mouse IL-6 is a single non-glycosylated polypeptide chain containing 188 amino acids.

Background: Interleukin-6 (IL-6) is a multifunctional protein that plays important roles in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by a variety of normal and transformed cells including T-cells, B-cells, monocytes/macrophages, fibroblasts, hepatocytes, keratinocytes, astrocytes, vascular endothelial cells, and various tumor cells. The production of IL-6 is upregulated by numerous signals including mitogenic or antigenic stimulation, LPS, calcium ionophore, IL-1, IL-2, IFN, TNF, PDGF, and viruses. IL-6 expression in monocytes is inhibited by IL-4 and IL-13.

Entrez Symbol:

Il6

Uniprot Name:

Interleukin-6

Uniprot ID:

P08505

Data PDF:

CRI130

Source:

E. coli

Molecular Weight:

~21.7 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 µm sterile filtered solution of PBS, pH 7.4.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is <0.02 ng/ml.

Endotoxin Level:

<1 EU/µg as determined by LAL method.

Amino Acid Sequence:

MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

The lyophilized protein is stable at 2-8°C. Upon receipt, store desiccated at -20°C. After reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.