Replaced by CRI001 (Recombinant Mouse Interferon-gamma)

Molecule:

IFN-gamma

Synonyms:

IFN-g, IFN-gamma, IFN-γ

Species:

Mouse

Cat. No.:

CR2041C pdf (datasheet)

Quantity/Size:

1 mg

Description:

Recombinant Mouse Interferon-gamma is a single non-glycosylated polypeptide chain containing 134 amino acids.
Background: Interferon gamma (IFN-gamma) is a soluble cytokine and a type II interferon. IFN-gamma is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the IFN-gamma receptor, which triggers a cellular response to viral and microbial infections. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-gamma induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-gamma augments TH1 cell expansion and may be required for TH1 cell differentiation.

Entrez Symbol:

Ifng

Uniprot ID:

P01580

Data PDF:

CR2041

Source:

E. coli

Molecular Weight:

~15.7 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 10 mM sodium phosphate + 50 mM sodium chloride, pH 7.5.

Purity:

>95% by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The biological activity is determined by a viral CPE assay using EMC virus on L929 cells.

Specific Activity:

>1 x 10e6 U/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis.

Amino Acid Sequence:

MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized protein is stable at room temperature for shipping purposes. Store desiccated at -20°C on receipt. After reconstitution, stable for ≤1 month at 2-8°C. For long term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.