Recombinant Mouse LIF

Molecule:

LIF

Synonyms:

Leukemia inhibitory factor, Lif

Species:

Mouse

Cat. No.:

CSI20150C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,000.00 / Special Price: $1,640.00 BUY

Description:

Recombinant Mouse LIF is a single non-glycosylated polypeptide chain containing 181 amino acids.

Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines, and promotion of megakaryocyte production in vivo

Entrez Symbol:

Lif

Uniprot Name:

Leukemia inhibitory factor

Uniprot ID:

P09056

Data PDF:

CSI20150

Source:

E. coli

Molecular Weight:

20 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % Tween-20.

Purity:

>98 % by SDS-PAGE

Biological Activity:

The ED50 as determined by the ability to inhibit proliferation of mouse M1 cells is typically < 0.03 ng/ml.

Specific Activity:

> 3.3 x 107 units/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10 mM acetic acid to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.