Leukemia inhibitory factor, Lif
Recombinant Mouse LIF is a single non-glycosylated polypeptide chain containing 181 amino acids.
Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines, and promotion of megakaryocyte production in vivo.
Leukemia inhibitory factor
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % Tween-20.
>98 % by SDS-PAGE
The ED50 as determined by the ability to inhibit proliferation of mouse M1 cells is typically < 0.03 ng/ml.
> 3.3 x 107 units/mg
≤1 EU/µg of protein by kinetic LAL analysis
Amino Acid Sequence:
MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F
Centrifuge vial prior to opening. Add sterile 10 mM acetic acid to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.