Recombinant Mouse VEGF 120

Molecule:

VEGFA

Synonyms:

VEGF-A, VPF, VEGF

Species:

Mouse

Cat. No.:

CSI20128C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,920.00 BUY

Description:

Recombinant Mouse VEGF 120 is a non-glycosylated homodimer containing two chains with 121 amino acids each.

Background: Vascular endothelial growth factor is a member of the PDGF/VEGF growth factor family. VEGF is a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. It also affects a number of other cell types (e.g. stimulation of monocyte/ macrophage migration, neurons, cancer cells, kidney epithelial cells). Alternative splicing results in variants having 120, 164, or 188 amino acids. Receptor tyrosine kinases (RTKs) Flt-1 and Flk-1 have been shown to bind VEGF with high affinity.

Data PDF:

CSI20128

Source:

E. coli

Molecular Weight:

~28.4 kDa

Formulation:

Lyophilized from a sterile-filtered solution with 0.1% Trifluoroacetic Acid (TFA).

Purity:

>95% by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The activity is determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) and is typically 1-5 ng/ml.

Specific Activity:

~1 × 106 U/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.