Recombinant Porcine IL-10

Molecule:

IL-10

Synonyms:

cytokine synthesis inhibitory factor, CSIF

Species:

Pig

Cat. No.:

CRI188A pdf (datasheet)

Quantity/Size:

10 μg

Price:

$450.00 BUY

Description:

Product: Recombinant porcine IL-10 is produced in E. coli and purified via sequential chromatography.

Entrez Symbol:

IL10

Uniprot Name:

Interleukin-10

Uniprot ID:

Q29055

Data PDF:

CRI188

Source:

E. coli

Molecular Weight:

18 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered (through a 0.22 micron filter) solution without carrier.

Purity:

>95% by SDS-PAGE analysis.

Biological Activity:

ED50 range: 10-20 ng/ml (As determined by MC/9 cell line proliferation assay with MTS detection).

Endotoxin Level:

<0.1 ng/�g

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of 0.1-1.0 mg/ml. The solution can then be further diluted in medium or buffered solution containing tissue culture grade carrier protein, such as PBS with 0.1-1.0% BSA.

Storage/Stability:

Lyophilized protein is stable at 2-4℃C for 1 year from date of receipt, but is preferably stored desiccated. After reconstitution, aliquot and store for >3 months at ?-20℃C. Avoid repeated freeze-thaw cycles.