Recombinant Porcine TNF-alpha

Molecule:

TNF-alpha/TNFSF1A

Synonyms:

TNFa, TNF-a, TNF-alpha, cachectin, tumor-necrosis factor, tumor necrosis factor alpha, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor ligand superfamily member 2

Species:

Pig

Cat. No.:

CRT107A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$215.00 BUY

Description:

Product: Recombinant porcine TNF-a is produced in E. coli and purified via sequential chromatography.

Entrez Symbol:

TNF

Uniprot Name:

Tumor necrosis factor

Uniprot ID:

P23563

Data PDF:

CRT107

Source:

E. coli

Molecular Weight:

17.1 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered (through a 0.22 micron filter) solution without carrier.

Purity:

>95% by SDS-PAGE analysis.

Biological Activity:

ED50 range: 0.001-0.015 ng/ml (As determined by the dose dependent cytotoxicity of porcine PK15 cells. Optimal concentration for individual application should be determined by a dose response assay).

Endotoxin Level:

<0.1 ng/�g

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of 0.1-1.0 mg/ml. The solution can then be further diluted in low endotoxin medium or buffered solution with heat inactivated FCS or tissue culture grade BSA.

Storage/Stability:

Lyophilized protein is stable at 2-4℃C for 1 year from date of receipt, but is preferably stored desiccated. After reconstitution, aliquot and store at ?-20℃C. Avoid repeated freeze-thaw cycles.

Application notes:

The optimal concentration should be determined for each specific application.