Recombinant Rat CNTF

Molecule:

CNTF

Synonyms:

ciliary neurotrophic factor , CNTF

Species:

Rat

Cat. No.:

CRC401B pdf (datasheet)

Quantity/Size:

25 μg

Price:

$425.00 BUY

Description:

Recombinant Rat Ciliary Neurotrophic Factor Background: Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 199 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL6, IL11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal transducing subunit in their receptor complexes.

Entrez Symbol:

Cntf

Uniprot Name:

Ciliary neurotrophic factor

Uniprot ID:

P20294

Data PDF:

CRC401

Source:

E. coli

Molecular Weight:

22.7 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized in 5 mM Sodium Acetate, pH 6.5.

Purity:

>98% by SDS-PAGE & silver stain

Biological Activity:

Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is typically 3-15 ng/ml.

Endotoxin Level:

<0.1 ng/οΨΒg of CNTF

Amino Acid Sequence:

AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM

Reconstitution:

Centrifuge vial prior to opening. The lyophilized CNTF is soluble in water and most aqueous buffers. The lyophilized protein should be reconstituted in PBS to a concentration of 0.1 mg/ml. This solution can be diluted water or other buffered solutions or

Storage/Stability:

The lyophilized protein, though stable at room temperature for three weeks, is best stored desiccated at -20οΨΑC. Reconstituted CNTF should be stored in working aliquots at -20οΨΑC. For long term storage of reconstituted protein, it is recommended that a