Recombinant Rat IGF-I

Molecule:

IGF-I

Synonyms:

Somatomedin C, IGF-I, IGFIA, IGF1, IGFI

Species:

Rat

Cat. No.:

CRI117C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,260.00 BUY

Description:

Background: The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250).

Entrez Symbol:

Igf1

Uniprot Name:

Insulin-like growth factor I

Uniprot ID:

P08025

Data PDF:

CRI117

Source:

E. coli

Molecular Weight:

~7.7 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 οΨΒm filtered concentrated solution in PBS, pH 7.4.

Purity:

>97% by RP-HPLC and SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using serum free human MCF-7 cells is less than 3 ng/ml.

Endotoxin Level:

<1 EU/οΨΒg as determined by LAL method.

Amino Acid Sequence:

GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to the vial to a concentration not less than 100 οΨΒg/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.

Storage/Stability:

The lyophilized protein is stable at 2-8οΨΑC. Upon receipt, store desiccated at -20οΨΑC. After reconstitution, the preparation is stable for up to one week at 2-8οΨΑC. For maximal stability, apportion the reconstituted preparation into working aliquots