Recombinant Rat IL-2

Molecule:

IL-2

Synonyms:

Interleukin-2, aldesleukin, T cell growth factor

Species:

Rat

Cat. No.:

CRI146C pdf (datasheet)

Quantity/Size:

1 mg

Price:

$1,895.00 BUY

Description:

Recombinant rat IL-2 is a single non-glycosylated polypeptide chain containing 136 amino acids.

Background: IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes.

Entrez Symbol:

Il2

Uniprot Name:

Interleukin-2

Uniprot ID:

P17108

Data PDF:

CRI146

Source:

E. coli

Molecular Weight:

15.6 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic acid (TFA).

Purity:

>95% by SDS-PAGE analysis.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 1 ng/ml.

Specific Activity:

> 1 × 106 U/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis.

Amino Acid Sequence:

MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAICQSI ISTMTQ

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10 mM HCl to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store lyophilized product at -20°C. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.