Recombinant Rat TNF-alpha/TNFSF1A

Molecule:

TNF-alpha

Synonyms:

Tnfa; RATTNF; TNF-alpha

Species:

Rat

Cat. No.:

CRT102C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,500.00 BUY

Description:

Recombinant rat TNF-alpha is a non-glycosylated protein, containing 157 amino acids.

Background: Tumor necrosis factor (TNF) is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/ TNFR1 and TNFRSF1B/ TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Entrez Symbol:

Tnf

Uniprot Name:

Tumor necrosis factor

Uniprot ID:

P16599

Data PDF:

CRT102

Source:

E. coli

Molecular Weight:

17.3 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 5 mM sodium phosphate and 50 mM sodium chloride, pH 7.5.

Purity:

≥95% as determined by SDS-PAGE.

Biological Activity:

Fully biologically active when compared to standard. The ED50 determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D, typically < 50 pg/ml.

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis.

Amino Acid Sequence:

MLRSSSQNSS DKPVVHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.