Recombinant Rhesus Macaque IL-1 beta

Molecule:

IL-1 beta

Synonyms:

interleukin 1, beta

Species:

Rhesus Macaque

Cat. No.:

CRI269B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Recombinant Rhesus Macaque IL-1 beta is a a single non-glycosylated polypeptide chain containing 153 amino acids.

Background: IL-1 beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. The 17 kDa mature Rhesus IL-1 beta shares 96% aa sequence identity with Human IL-1 beta.

Data PDF:

CRI269

Source:

E. coli

Molecular Weight:

~17.3 kDa

Formulation:

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Purity:

>98% by SDS-PAGE and HPLC analyses

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of D10.G4.1 mouse helper T cells is typically 3-10 pg/mL.

Endotoxin Level:

Less than 1EU/µg of rRhIL-1 beta as determined by LAL method.

Amino Acid Sequence:

APVRSLHCTL RDAQLKSLVM SGPYELKALH LQGQDLEQQV VFSMSFVQGE ESNDKIPVAL GLKAKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTRGG QDITDFTMQF VSS

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be divided into working aliquots and stored at -80°C. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

The lyophilized protein is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. After reconstitution, the protein is stable for 1 week at 2-4°C. For long term storage, aliquot and freeze at -80°C. Avoid repeated freeze-thaw cycles.