Recombinant Rhesus Macaque IL-6

Molecule:

IL-6

Synonyms:

Interleukin 6, interferon beta 2

Species:

Rhesus Macaque

Cat. No.:

CRI271A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$190.00 BUY

Description:

Recombinant Rhesus Macaque IL-6 is a single non-glycosylated polypeptide chain containing 186 amino acids.

Background: IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130.

Entrez Symbol:

IL6

Data PDF:

CRI271

Source:

E. coli

Molecular Weight:

~21.1 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent mouse 7TD1 cells is <0.1 ng/ml.

Endotoxin Level:

<1 EU/μg of Recombinant Rhesus Macaque Interleukin-6 as determined by LAL method

Amino Acid Sequence:

MAPVLPGEDS KNVAAPHSQP LTSSERIDKH IRYILDGISA LRKETCNRSN MCESSKEALA ENNLNLPKMA EKDGCFQSGF NEDTCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PEPTTNASLL TKLQAQNQWL QDMTTHLILR SFKEFLQSNL RALRQM

Reconstitution:

Centrifuge vial prior to opening to bring contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

The lyophilized protein is stable at 2-8°C. Upon receipt, store desiccated at -20°C. After reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.