Synonyms:
Trypsin/factor XIIA inhibitor, Hageman factor inhibitor, CHF1
Description:
Corn Trypsin Inhibitor (CTI) is a highly specific inhibitor of trypsin and Factor XIIa that does not affect Factors VIIa, IXa, Xa, or thrombin. CTI forms a one-to-one molar complex with target proteases and is useful for distinguishing between the intrinsic and extrinsic coagulation cascades. CTI is extracted from high quality sweet corn meal into a physiologic buffer then purified by a combination of gel filtration, ion-exchange, and affinity chromatography. The final CTI preparation appears as a single band by SDS-PAGE analysis under both reducing and non-reducing conditions. Preparations of CTI are tested for the ability to prolong the aPTT assay without affecting the PT assay.
Concentration:
> 1 mg/ml, lot specific
Uniprot Name:
Trypsin/factor XIIA inhibitor
Molecular Weight:
12.5 kDa
Formulation:
20 mM Tris-HCl, 0.15 M NaCl, pH 7.4
Purity:
> 95% by SDS-PAGE analysis
Specific Activity:
>10 U/mg, 1 unit is defined as the amount of CTI required to double the aPTT of normal human plasma.
Storage/Stability:
Store at -80°C
