Cynomolgus Monkey IgG ELISA kit

Molecule:

IgG

Species:

Cynomolgus Monkey

Cat. No.:

CKK001A pdf (datasheet)

Quantity/Size:

1 x 96 tests

Price:

$560.00 BUY

Description:

Intended Use: This cynomolgus macaque (Macaca fascicularis) monkey Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total IgG antigen in cyno monkey serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgG subclasses.

Background: IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.

Assay Principle: Cynomolgus monkey IgG will bind to the monoclonal capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled monoclonal anti-Cynomolgus IgG primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Cynomolgus monkey IgG. Color development is proportional to the concentration of IgG in the samples.

Specifications:

  • Sensitivity: 0.077 ng/ml
  • Specificity: Total cynomolgus macaque (Macaca fascicularis) IgG (Does not distinguish IgG subclasses.)
  • Range: 0.1-100 ng/ml
  • Sample-type: Serum, plasma, hybridoma cell supernatants, ascites or other biological fluids
  • Cross-reactivity: Pooled normal plasma from Rhesus monkey was assayed and significant cross-reactivity was observed. Pooled normal plasma from human, mouse, rat, dog, sheep, pig and rabbit was assayed and no significant cross-reactivity was observed.

Reagents Provided:

  • 96-well microtiter strip plate coated with anti-Cynomolgus Monkey IgG antibody, dried and blocked on well surface
  • Wash buffer concentrate (10x)
  • Cynomolgus Monkey IgG standard, lyophilized
  • Biotinylated anti-Cynomolgus Monkey IgG primary antibody, lyophilized
  • Horseradish peroxidase-conjugated Streptavidin, concentrated
  • TMB substrate solution

Data PDF:

CKK001A

Storage/Stability:

All kit components must be stored at 4°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 4°C. Kit should be used no later than the expiration date.

Application:

ELISA