Human A431 Cell Lysate

Species:

Human

Cat. No.:

PX308A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$160.00 BUY

Description:

A431 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The A431 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Concentration:

2.0 mg/ml

Data PDF:

PX308

Formulation:

Supplied in SDS sample buffer containing 50 mM DTT.

Storage/Stability:

Store at 2-8°C for 6 months of continuous use. Store in working aliquots at -80°C for up to 1 year. Avoid repeated freeze-thaw cycles.

Application notes:

Centrifuge vial before use. Lysate is ready to load on SDS -PAGE for Western blotting. 20 µg per lane is recommended for a mini gel. The optimal concentration should be determined for each specific application.

Application:

WB