Human Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid mAb

Synonyms:

Nucleoprotein, Nucleocapsid protein, NC, Protein N

Species:

SARS-CoV-2

Cat. No.:

CPC518A pdf (datasheet)

Quantity/Size:

50 µg

Price:

$390.00 BUY

Concentration:

1.0 mg/ml

Description:

Recombinant Human anti-SARS-CoV-2 Nucleocapsid Protein is expressed in XtenCHO.

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Uniprot Name:

Nucleoprotein

Uniprot ID:

P0DTC9

Specificity:

Recognizes SARS-CoV-2 Nucleocapsid protein

Host Species:

Human

Immunogen:

Recombinant SARS-CoV-2 Nucleocapsid protein

Isotype:

IgG

Formulation:

Sterile-filtered PBS, pH 7.5 preservative free.

Purification:

Protein A affinity chromatography

Application:

ELISA, WB

Application notes:

ELISA: suggested dilution 1:5,000 - 1:10,000
Western blot: suggested dilution 1:1,000 - 1:2,000

Storage/Stability:

Stable at 2-8°C for 1 week or for up to 1 year at -20°C to -80°C. It is recommended to prepare working aliquots of undiluted product and store -20°C to -80°C.