Human Anti-SARS-CoV-2 (2019-nCoV) Spike S1, Clone H4, Neutralizing mAb

Synonyms:

Spike glycoprotein, Spike S1 subunit, S glycoprotein

Species:

SARS-CoV-2

Cat. No.:

CPC521B pdf (datasheet)

Quantity/Size:

100 µg

Price:

$490.00 BUY

Concentration:

1.0 mg/ml

Description:

Recombinant Human anti-SARS-CoV-2 Spike S1, Clone H4 is expressed in XtenCHO.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID -19). The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. Spike glycoprotein is cleaved into the following 3 chains, Spike protein S1, Spike protein S2, Spike protein S2'. Spike protein S1 attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Surface glycoprotein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Uniprot Name:

Spike glycoprotein

Uniprot ID:

P0DTC2

Specificity:

Recognizes SARS-CoV-2 Spike S1 protein

Host Species:

Human

Immunogen:

Recombinant SARS-CoV-2 Spike S1 protein

Isotype:

IgG1

Clone:

H4

Formulation:

Sterile-filtered PBS, pH 7.5 preservative free.

Purification:

Protein A affinity chromatography

Application:

ELISA, Neutralizing , WB

Application notes:

ELISA: 1:5,000 - 1:10,000
Western blot: suggested dilution 1:1,000 - 1:2,000

Storage/Stability:

Stable at 2-8°C for 1 week or for up to 1 year at -20°C to -80°C. It is recommended to prepare working aliquots of undiluted product and store -20°C to -80°C.