Human Brain Tissue Lysate

Species:

Human

Cat. No.:

PX335B pdf (datasheet)

Quantity/Size:

0.5 mg

Price:

$415.00 BUY

Description:

Human brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl + 50 mM Tris-HCl, pH 7.4 + 1 mM ethylenediaminetetraacetic acid + 1 mM phenylmethylsulfonyl fluoride + 1% Triton X-100 + 1% sodium deoxycholic acid + 0.1% sodium dodecylsulfate + 5 µg/ml of aprotinin + 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Concentration:

2.0 mg/ml

Data PDF:

PX335

Source:

Human Brain

Formulation:

Liquid in SDS sample buffer with 5% β-mercaptoethanol

Storage/Stability:

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year. Avoid repeated freeze-thaw cycles.

Application notes:

Centrifuge vial before use. Ready to load on SDS-PAGE. 20 µg per lane is recommended for a mini gel. The optimal concentration should be determined by the user for each specific application.