Human Caco-2 Cell Lysate

Species:

Human

Cat. No.:

PX311B pdf (datasheet)

Quantity/Size:

0.5 mg

Price:

$395.00 BUY

Description:

Caco-2 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with protein assay. The Caco-2 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Concentration:

2.0 mg/ml

Data PDF:

PX311

Formulation:

Liquid in 1x SDS sample buffer containing 5% beta-mercaptoethanol.

Storage/Stability:

Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -80˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

Application notes:

Centrifuge vial before use. Caco-2 cell lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 μg to 20 μg per lane for a mini gel. The optimal concentration should be determined by the user for each specific application.

Application:

WB