Human sCD14 ELISA Kit

Molecule:

CD14

Synonyms:

myeloid cell-specific leucine-rich glycoprotein

Species:

Human

Cat. No.:

CKH114 pdf (datasheet)

Quantity/Size:

1 Plate

Price:

$845.00 BUY

Description:

The human sCD14 kit has been developed for the quantitative measurement of natural and recombinant human CD14 in serum, plasma and culture medium.

The sCD14 kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A mixture of two monoclonal antibodies specific for sCD14 is pre-coated to the modules of the plate. In the first step the pre-coated modules will be incubated with the antigen (standard or sample). During this incubation, human CD14 is captured by solid bound antibody. Unbound material present in the sample will be removed by washing. Next, a POD-labelled monoclonal antibody specific for sCD14 is incubated. The detection step includes TMB as chromogen. The enzyme reaction is stopped by the addition of 0.25 mol sulphuric acid and the absorption is measured at 450 nm with a spectrophotometer. A standard curve is obtained by plotting the absorptions versus the corresponding concentrations of the known standards. The human CD14 concentration of samples with unknown concentrations, which are run concurrently with the standards, can be determined by the standard curve.

Entrez Symbol:

CD14

Uniprot Name:

Monocyte differentiation antigen CD14

Uniprot ID:

P08571

Data PDF:

CKH114

Kit Plates:

Precoated plates

Application:

ELISA