Anti-Human CD3 / CD28 Stimulus for Human PBMC

Cat. No.:

CMC443 pdf (datasheet)

Quantity/Size:

50 tests

Price:

$240.00 BUY

Description:

To be used as a polyclonal T cell activator in the human cytokine ELISPOT analyses. The vial contains two antibodies specific for human CD3 and CD28. A variety of different cytokines are elicited by this antibody combination including IFN-γ, IL-2, IL-4, IL-5, IL-10 and IL-13.

Data PDF:

CMC443

Formulation:

Prior to lyophilization: 0.5 ml sterile-filtered RPMI with 10% FCS, 125 mM trehalose. Preservative free.

Reconstitution:

Reconstitute the lyophilized contents by injecting 500 µl sterile distilled water into the vial.

Application:

ELISPOT

Application notes:

Recommended working concentration is 100x in culture medium containing > 10e4 human PBMC/ml.

Storage/Stability:

Store at 2-8°C or in working aliquots at -80°C.