Anti-Human CD3 / CD28 Stimulus for Human PBMC

Cat. No.:

CMC443 pdf (datasheet)

Quantity/Size:

50 tests

Price:

$240.00 BUY

Description:

To be used as a polyclonal T cell activator in the human cytokine ELISPOT analyses. The vial contains two antibodies specific for human CD3 and CD28. A variety of different cytokines are elicited by this antibody combination including IFN-γ, IL-2, IL-4, IL-5, IL-10 and IL-13.

Data PDF:

CMC443

Formulation:

Prior to lyophilization: 0.5 ml sterile-filtered RPMI with 10% FCS, 125 mM trehalose. Preservative free.

Reconstitution:

Reconstitute the lyophilized contents by injecting 500 µl sterile distilled water into the vial. Mix the solution gently for approximately 15 seconds and allow it to stand for 2 minutes at room temperature. Avoid vigorous shaking. Do not use if the solution is or has become turbid.

Application:

ELISPOT

Application notes:

Recommended working concentration is 100x in culture medium containing > 104 human PBMC/ml.

Storage/Stability:

Store as supplied at 2-8°C until expiration date. After reconstitution as directed, stable for at least 1 month at 2-8°C when kept sterile. However, it is strongly recommended to divide the reconstituted product into small aliquots for single use. These aliquots should be stored at -80°C. Under these conditions the product is stable for at least 1 year.