Anti-Human CD3 / CD28 Stimulus for Human PBMC

Cat. No.:

CMC443 pdf (datasheet)


50 tests


$240.00 BUY


To be used as a polyclonal T cell activator in the human cytokine ELISPOT analyses. The vial contains two antibodies specific for human CD3 and CD28. A variety of different cytokines are elicited by this antibody combination including IFN-γ, IL-2, IL-4, IL-5, IL-10 and IL-13.

Data PDF:



Prior to lyophilization: 0.5 ml sterile-filtered RPMI with 10% FCS, 125 mM trehalose. Preservative free.


Reconstitute the lyophilized contents by injecting 500 µl sterile distilled water into the vial. Mix the solution gently for approximately 15 seconds and allow it to stand for 2 minutes at room temperature. Avoid vigorous shaking. Do not use if the solution is or has become turbid.



Application notes:

Recommended working concentration is 100x in culture medium containing > 104 human PBMC/ml.


Store as supplied at 2-8°C until expiration date. After reconstitution as directed, stable for at least 1 month at 2-8°C when kept sterile. However, it is strongly recommended to divide the reconstituted product into small aliquots for single use. These aliquots should be stored at -80°C. Under these conditions the product is stable for at least 1 year.