Human Colon Tissue Lysate

Species:

Human

Cat. No.:

PX466A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$175.00 BUY

Description:

Human colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM TrisHCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Concentration:

2.0 mg/ml

Data PDF:

PX466

Formulation:

Colon lysate is supplied in 1x SDS sample buffer containing 50 mM DTT.

Storage/Stability:

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year. in working aliquots at -80°C for up to 1 year.

Application notes:

The human colon tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

Application:

WB