Human Factor X Total Antigen Assay ELISA Kit





Cat. No.:

CSI20521A pdf (datasheet)


1 Kit


$735.00 BUY


Factor X is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor Xa. Factor X is activated by Factor IXa in complex with Factor VIII, calcium and phospholipids during the intrinsic pathway and by Factor VIIa in complex with Tissue Factor, calcium and phospholipids during the extrinsic pathway of the coagulation cascade. The sensitive quantitative measurement of total human Factor X antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Human Factor X and Xa will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, polyclonal anti-human Factor X primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor X present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor X. Color development is proportional to the concentration of Factor X in the samples.

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Kit Plates:

96-well precoated strip plate


Store all reagents at 2-8°C.