Human Heart Ventricle (LT) Membrane Lysate

Species:

Human

Cat. No.:

CLH003 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$595.00 BUY

Description:

Membrane Protein Lysate of Normal Human Heart Left Ventricle. Human heart ventricle (left) tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human heart ventricle (left) tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The membrane protein is provided in a buffer including HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated heart ventricle (left) tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated heart ventricle (left) tissue membrane protein is then Western analyzed by either GAPDH or b-actin antibody to confirm there is no signal or very weak signal.

Concentration:

≥2 mg/ml

Data PDF:

CLH003

Formulation:

Frozen liquid containing HEPES pH, 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40.

Storage/Stability:

Store at 2-8°C for 6 months of continuous use. Store in working aliquots at -80°C for up to 1 year. Avoid repeated freeze-thaw cycles.

Application notes:

Centrifuge vial before use. Lysate is ready to load on SDS -PAGE for Western blotting. 10-20 µg per lane is recommended for a mini gel. The optimal concentration should be determined for each specific application.

Application:

WB