Human IgG B cell ELISPOT kit w/o plates

Molecule:

IgG

Cat. No.:

CKH338 pdf (datasheet)

Quantity/Size:

5 X 96 tests, plates not included

Price:

$1,330.00 BUY

Description:

The B cell ELISPOT (Enzyme-linked Immunospot) assay has been designed to identify and enumerate individual antibody secreting cells (ASC) in single cell suspensions of (most commonly) peripheral blood mononuclear cells (PBMC). The traditional method to monitor a B cell response generated after immunization or infection is to quantify specific antibody titers in serum by ELISA. The ELISA method is a straightforward method to measure serum antibody titers. However, the assay does not provide any information about the number and location of the ASCs. The B cell ELISPOT assay has proven particularly useful to identify and determine the number of individual ASCs in single cell suspensions. Memory B cells, which have a long lifespan, play a central role in the humoral immune response. Under natural conditions these B cells do not proliferate or produce antibodies until they are activated by re-exposure to a specific antigen. While being inactive, the presence of these cells can not be determined by ELISA. A major advantage of the B cell ELISPOT is its ability to activate ex vivo antigen-specific memory B cells, whereafter they can be detected. The B cell ELISPOT is the assay of choice to determine the magnitude and longevity of (vaccine-induced) protection against a certain infection. The B cell ELISPOT assay is useful in different fields of biomedical research including vaccine development, infection research, drug treatment, autoimmune diseases and allergy.

Data PDF:

CKH338

Kit Plates:

PVDF plates not included