Human IgG ELISA Kit

Species:

Human

Cat. No.:

CS222B pdf (datasheet)

Quantity/Size:

5 x 96 tests each

Price:

$1,470.00 BUY

Description:

Background: IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.

Assay Principle: Human IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgG antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human IgG. Color development is proportional to the concentration of IgG in the samples.

Reagents Provided
96-well microtiter strip plate coated with anti- Human IgG antibody, blocked and dried on well surface
Wash Buffer Concentrate (10x)
Human IgG standard, lyophilized
Anti-Human IgG primary antibody, lyophilized polyclonal antibody
Horseradish peroxidase-conjugated Streptavidin, concentrated
TMB substrate solution

Data PDF:

CS222

Kit Plates:

96-well microtiter strip plate coated with anti- Human IgG antibody, blocked and dried on well surface.

Storage/Stability:

All kit components must be stored at 2-8°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 2-8°C. Kit should be used no later than the expiration date.