Description:
The Human IL17A ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL17A in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Human IL17A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL17A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human IL17A antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL17A bound.
