An economical alternative to the PeliKine human IL-6 ELISA (M1916), the PeliPair Human IL-6 reagent set consist of matched antibodies and Standard sufficient for 19 ELISA plates to quantify human IL-6 in cell supernatants over the range 0.6 - 450 pg/ml.

A protocol is included which recommends starting concentrations for coating and biotinylated antibodies, and directions for preparation of buffers and solutions. We suggest using High Performance ELISA buffer (M1940) and Streptavidin-HRP conjugate (M2032) components. As an alternative to making buffers and solutions, a Reagent Tool Set (M1980) may be purchased separately, which includes all buffers and solutions to construct an ELISA.