Human Kidney Tissue Lysate

Species:

Human

Cat. No.:

PX338A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$175.00 BUY

Description:

Human kidney lysate was prepared by homogenization in modified RIPA buffer: 150 mM Sodium Chloride + 50 mM Tris-HCl, pH 7.4 + 1 mM EDTA + 1 mM PMSF + 1% Triton X -100, 1% Sodium Deoxycholic Acid + 0.1% SDS + 5 μg/mL Aprotinin + 5 μg/mL Leupeptin. Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer: 50 mM Tris-HCl pH 6.8 + 12.5% Glycerol + 1% SDS + 0.01% Bromophenol Blue) + 5% BME.

Concentration:

2 mg/ml

Data PDF:

PX338

Formulation:

Supplied as whole cell lysate in 1 x SDS sample buffer containing 5% betamercaptoethanol.

Storage/Stability:

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year.

Application notes:

Centrifuge vial before use. Human Kidney Tissue Lysate Normal is ready to load on SDS -PAGE for Western blotting, 10-20 µg per lane is recommended for mini gel. The optimal concentration should be determined for each specific application.

Application:

WB