Human Kidney Tissue Lysate, Normal

Species:

Human

Cat. No.:

PX354 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$250.00 BUY

Description:

Human tissue lysate has not been subjected to heat denaturation or reducing conditions. This allows the tissue lysate to be used in a variety of applications; to study protein protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and western blotting for the detection of specific protein targets.

Cell Sciences now offers a Human Tumor tissue lysate collection consisting of a diverse range of cancer pathologies from over 20 human organs, defined by cell type, tumor grade and stage.  A majority of our Tumor lysates are available as matched sets, consisting of the Tumor lysate and adjacent Normal Tissue lysate for easy comparison studies and accurate biomarker identification. 

Please inquire regarding a matched set with a specific pathology. 

Concentration:

1.0 mg/ml

Data PDF:

PX354

Source:

Human

Formulation:

PBS, pH 7.4, 0.1% SDS, 0.25% Na deoxycholate, 1 mM EDTA, 1 mM Na3VO4, 1 mM NaF, 1 µg/ml Aprotinin, 1 µg/ml Pepstatin-A, 1 µg/ml Leupeptin, 1 mM PMSF

Storage/Stability:

Store at -80°C for up to 1 year.

Application notes:

For use in 1D and 2D gel electrophoresis, western blot, the addition of a denaturing gel loading buffer with reducing agents may be required.

Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysate in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.

Application:

ELISA, RIA, WB