LONG®R3 IGF-1 is an analog of IGF-1 which has been engineered for improved bioavailability compared to IGF-1. It binds to and activates the Type I IGF-1 receptor, leading to improved cell growth and productivity. Quantitation of LONG®R3 IGF-1 is important when developing cell culture processes in order to optimize the concentration of LONG®R3 IGF-1 used and feeding strategies. Quantitation is also important when developing a purification process, in order to demonstrate clearance.

This ELISA kit has been developed for the quantitation of LONG®R3 IGF-1 in media and drug substance samples. As shown in Figure 1, the plate is coated with a monoclonal antibody to LONG®R3 IGF-1 and then incubated with samples. A second biotinylated detection antibody is added, followed by Streptavidin-HRP. The plate is incubated with TMB Substrate for color development and stopped with sulfuric acid. The signal is proportional to the amount of LONG®R3 IGF-1 in samples and standards.

Range: 0.3 - 40 ng/ml

Sensitivity: 0.3 ng/ml

Reproducibility: Intra-Assay: CV≤12%

S/N ratio: Cell culture supernatants and media

Recovery: 70-130% for 20, 2.5, and 1.25 ng/ml sample concentrations

Correlation Coefficient: ≥0.985 for 4-parameter logistical standard curve fit. \

Reagents Included:

• Microplate 96-wells pre-coated with Capture Antibody
• LoBind Eppendorf tubes
• Diluent
• LONG® R3 IGF-1 Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP
• TMB Substrate