LONG®R3 IGF-1 is an analog of IGF-1 which has been engineered for improved bioavailability compared to IGF-1. It binds to and activates the Type I IGF-1 receptor, leading to improved cell growth and productivity. Quantitation of LONG®R3 IGF-1 is important when developing cell culture processes in order to optimize the concentration of LONG®R3 IGF-1 used and feeding strategies. Quantitation is also important when developing a purification process, in order to demonstrate clearance.
This ELISA kit has been developed for the quantitation of LONG®R3 IGF-1 in media and drug substance samples. As shown in Figure 1, the plate is coated with a monoclonal antibody to LONG®R3 IGF-1 and then incubated with samples. A second biotinylated detection antibody is added, followed by Streptavidin-HRP. The plate is incubated with TMB Substrate for color development and stopped with sulfuric acid. The signal is proportional to the amount of LONG®R3 IGF-1 in samples and standards.
Range: 0.3 - 40 ng/ml
Sensitivity: 0.3 ng/ml
Reproducibility: Intra-Assay: CV≤12%
S/N ratio: Cell culture supernatants and media
Recovery: 70-130% for 20, 2.5, and 1.25 ng/ml sample concentrations
Correlation Coefficient: ≥0.985 for 4-parameter logistical standard curve fit. \
Reagents Included:
- Microplate 96-wells pre-coated with Capture Antibody
- LoBind Eppendorf tubes
- Diluent
- LONG® R3 IGF-1 Standard
- Biotinylated Detection Antibody
- Streptavidin-HRP
- TMB Substrate