LONG®R3 IGF-1 is an analog of IGF-1 which has been engineered for improved bioavailability compared to IGF-1. It binds to and activates the Type I IGF-1 receptor, leading to improved cell growth and productivity. Quantitation of LONG®R3 IGF-1 is important when developing cell culture processes in order to optimize the concentration of LONG®R3 IGF-1 used and feeding strategies. Quantitation is also important when developing a purification process, in order to demonstrate clearance.

This ELISA kit has been developed for the quantitation of LONG®R3 IGF-1 in media and drug substance samples. As shown in Figure 1, the plate is coated with a monoclonal antibody to LONG®R3 IGF-1 and then incubated with samples. A second biotinylated detection antibody is added, followed by Streptavidin-HRP. The plate is incubated with TMB Substrate for color development and stopped with sulfuric acid. The signal is proportional to the amount of LONG®R3 IGF-1 in samples and standards.

Range:   0.31 - 40 ng/ml

Sensitivity:   0.3 ng/ml   

Cross Reactivity:   Limited reactivity to native or recombinant IGF-1.  

                            No reactivity to insulin