Human Lung Tissue Lysate

Species:

Human

Cat. No.:

PX340A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$175.00 BUY

Description:

Human Lung Tissue Lysate was prepared by homogenization in a modified RIPA buffer (150 mM Sodium Chloride, 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 1 mM PMSF, 1% Triton X-100, 1% Sodium Deoxycholic Acid, 0.1% SDS, 5 μg/mL Aprotinin, 5 μg/mL Leupeptin.) Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.

The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.01% Bromophenol Blue) containing 50 mM DTT.

Concentration:

2.0 mg/ml

Data PDF:

PX340

Formulation:

Supplied in SDS sample buffer containing 50 mM DTT.

Storage/Stability:

Store at 2-8oC for continuous use. Store in working aliquots at -80°C for up to 1 year. Avoid repeated freeze-thaw cycles.

Application notes:

Centrifuge vial before use. Human Lung Tissue Lysate is ready to load on SDS -PAGE for Western blotting. 20 µg per lane is recommended for a mini gel. The optimal concentration should be determined for each specific application.

Application:

WB