Human MMP-2 ELISA Kit

Species:

Human

Cat. No.:

CKH340 pdf (datasheet)

Quantity/Size:

1 x 96 tests

Price:

$575.00 BUY

Description:

The Cell Sciences® Human MMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-2 in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended as anticoagulants for use in this assay due to their chelating properties), cell culture supernates and urine. This assay employs an antibody specific for human MMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Kit Plates:

96-well precoated strip plate

Storage/Stability:

Store all reagents at 2-8°C or -20°C. Avoid freeze thaw cycles.