Description:
The Cell Sciences® Human MMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is
an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of
human MMP-2 in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA
and Citrate are not recommended as anticoagulants for use in this assay due to their
chelating properties), cell culture supernates and urine. This assay employs an antibody
specific for human MMP-2 coated on a 96-well plate. Standards and samples are pipetted
into the wells and MMP-2 present in a sample is bound to the wells by the immobilized
antibody. The wells are washed and biotinylated anti-human MMP-2 antibody is added.
After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is
pipetted to the wells.
The wells are again washed, a TMB substrate solution is added to the wells and color
develops in proportion to the amount of MMP-2 bound. The Stop Solution changes the
color from blue to yellow, and the intensity of the color is measured at 450 nm.
