Human Ovary Tissue Lysate

Species:

Human

Cat. No.:

PX467A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$175.00 BUY

Description:

Human ovary tissue lysate was prepared by homogenization in a Lysis Buffer (10 mM HEPES, pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors) Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Concentration:

2.0 mg/ml

Data PDF:

PX467

Source:

Human

Formulation:

Ovary tissue lysate is supplied in SDS sample buffer containing 50 mM DTT.

Storage/Stability:

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year.

Application notes:

Centrifuge vial before use. The human ovary tissue lysate is ready to load on SDSPAGE for Western blotting. It is recommended to load 10 μg to 20 μg per lane for mini gel.

Application:

WB