Human Ovary Tissue Lysate

Species:

Human

Cat. No.:

PX467A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$175.00 BUY

Description:

Ovary tissue lysate was prepared by homogenization in a Lysis Buffer (10 mM HEPES, pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors) Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.

The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Concentration:

2.0 mg/ml

Data PDF:

PX467

Source:

Human

Formulation:

Ovary lysate is supplied in SDS sample buffer containing 50 mM DTT.

Storage/Stability:

Store at 2-8˚C for continuous use. For extended storage, store working aliquots at -80˚C for up to 1 year. Avoid repeated freeze/thaw cycles.

Application notes:

Centrifuge vial before use. The human ovary tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 μg to 20 μg per lane for mini gel.

Application:

WB