Human Perforin-1 ELISA Kit

Molecule:

Perforin-1

Synonyms:

cytolysin, lymphocyte pore forming protein, lymphocyte pore-forming protein, P1, PFP, FLH2, PFN1, HPLH2

Species:

Human

Cat. No.:

CKH331 pdf (datasheet)

Quantity/Size:

5 Plates

Price:

$850.00 BUY

Description:

The ELISA assay is a simple and sensitive sandwich immunoassay for the determination of perforin-1 levels in biological fluids such as cell culture supernatant, plasma or serum. The assay utilizes a coating antibody specific for perforin-1 coated on the wells of a 96-well microtiter plate. The wells are washed and blocked. Standards and samples are added to the wells and any perforin-1 present binds to the immobilized coating antibody. After washing off excess/unbound materials, the bound perforin-1 is allowed to associate with a biotinylated detection antibody. The wells are washed again and a streptavidin-HRP (SPP) conjugate is added to the antibody-antigen-antibody complex. After another wash, a chromogenic substrate (TMB) is introduced, which produces a blue-colored product of which the intensity is related to the amount of perforin-1 in the sample.

Entrez Symbol:

PRF1

Uniprot Name:

Perforin-1

Uniprot ID:

P14222