Human

PX325A pdf (datasheet)

100 μg

$190.00 BUY

Raji cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The MCF7 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

2 mg/ml

PX325

Lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.

Store in working aliquots at -80°C for up to 1 year.

Western blot: Centrifuge vial before use. Recommended 10 to 20 µg per lane for mini gel.

WB